Restriction Enzymes
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I FLTRD-25MG
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
New England Biolabs, Inc. pBR322 DNA-BstNI Digest – 0.25 ml
The BstNI Digest of pBR322 DNA yields 6 fragments suitable for use as molecular weight standards for agarose gel electrophoresis. Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.
New England Biolabs, Inc. HindIII – 10000 units
A restriction endonuclease that recognizes the sequence A^AGCT_T.
New England Biolabs, Inc. EcoNI – 1000 units
A restriction endonuclease that recognizes the sequence CCTNN^N_NNAGG.
New England Biolabs, Inc. PmlI – 2000 units
A restriction endonuclease that recognizes the sequence CAC_^GTG.
New England Biolabs, Inc. BfaI – 500 units
A restriction endonuclease that recognizes the sequence C^TA_G.
New England Biolabs, Inc. AvrII – 500 units
A restriction endonuclease that recognizes the sequence C^CTAG_G.
New England Biolabs, Inc. Eco53kI – 1000 units
A restriction endonuclease that recognizes the sequence GAG_^CTC.
New England Biolabs, Inc. WarmStart Nt.BstNBI-1000 u
WarmStart Nt.BstNBI is a site specific nicking endonuclease cloned fromBacillus Stereothermophilus. Restriction Enzyme Cut Site GAGTC(4/-5). It is formulated with a reversibly-bound aptamer which inhibits its nicking activity at temperatures below 40C. WarmStart Nt.BstNBI cleaves only one strand of DNA on a double-stranded DNA substrate. The nicking endonuclease catalyzes a single strand break 4 bases beyond the 3side of the recognition sequence.
New England Biolabs, Inc. SacI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G_AGCT^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.